Semiautomated detection of human papillomavirus DNA of high and low oncogenic potential in cervical smears.

نویسندگان

  • G Terry
  • L Ho
  • A Szarewski
  • J Cuzick
چکیده

Detection of DNA from human papillomaviruses (HPV) of high and intermediate oncogenic risk in cervical smears may predict the presence of cervical cancer or may indicate precancerous changes. Here we describe a semiautomated polymerase chain reaction system for the detection and classification of HPV DNA that is present in clinically significant amounts in routine cervical scrapes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Failure to Demonstrate the Role of High Risk Human Papilloma Virus in Epithelial Ovarian Cancer

Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, some studies did not detect HPV DNA in ovarian carcinoma tissues. In this articl...

متن کامل

Familial porphyria cutanea tarda.

Semiautomated Detection of Human Papifiomavirus DNA of High and Low Oncogenic Potential in Cervical Smears. George Terry, Linda Ho, Anne Szarewski, Jack Cuzick 1890 Immunoassay with Recombinant Trypanosoma cruzi Antigens Potentially Useful for Screening Donated Blood and Diagnosing Chagas Disease. Ana C. Pastini, Sergio R. Iglesias, Valentina C. Carri carte, Marcelo E. Guerin, Daniel 0. Sanchez...

متن کامل

Cytotoxic T-Cell Markers and Cytokines in Human Papillomavirus 16

Background and Aim: Cervical cancer is the fourth main cause of mortality among women, and annually about half a million new cases are detected in developed countries. Based on oncological studies, human papillomavirus (HPV) is classified into two categories: high-risk type and low-risk type, and most cases are related to the high-risk type of human papillomavirus. HPV 16 and 18 are among the m...

متن کامل

Detection of Human Papillomavirus DNA by PCR in Esophageal Squamous Cell Carcinoma from Turkmen Sahra, North-East of Iran

Human papillomavirus (HPV) DNA has been identified in esophageal carcinomas. However, the incidence of HPV varies significantly in different geographical locations. In this study, neoplasms from Turkmen Sahra, a region in Golestan province in northeast part of Iran, with a high incidence of squamous cell carcinoma were analyzed for the presence of HPV DNA. Turkmen Sahra is located in the cancer...

متن کامل

DNA-EIA to detect high and low risk HPV genotypes in cervical lesions with E6/E7 primer mediated multiplex PCR.

BACKGROUND Oncogenicity of human papillomavirus (HPV) DNA in premalignant and malignant uterine cervical diseases is mainly induced by E6/E7 open reading frame (ORF). The presence of an oncogenic HPV DNA may be a diagnostic marker for the detection of cytologically negative smears. AIMS To evaluate an original polymerase chain reaction enzyme immunoassay (PCR-EIA) for the detection and typing...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 40 10  شماره 

صفحات  -

تاریخ انتشار 1994